Mushy Mayhem

[terrancefartbubbler]

Yep I have, just the cheap brown rice, Coles does a box of 4 or 5 pouches. I vacuumed the room I planned doing it in, sprayed Glen20 in the room also and wiped down the table and 50l storage container, placed the accessories under the tub on table. Waited 20 mins or so with door shut then took a shower, went back in with wet hair and sprayed scissors, syringe hands with alcohol,
Did everything very slowly under the container leaning a bit over the desk so I could fit hands through if that makes sense, had an 80% success rate

How many cc's do you inject in rice pouches?

 

[Goonie Goat]

How many cc's do you inject in rice pouches?

I'd just do 5 drops. You are using liquid cultures aren't you? That's plenty. Shake it up well beforehand

 

[terrancefartbubbler]

Yep using a LC, my Tasmanian strain that I inoculated on the 7th and then again on the 17th has finally started growing mycelium thought it might of been dead but just really slow

 

[terrancefartbubbler]

Can anyone tell me if this is mould or mycelium? It is growing off a ridge of mycelium not sure if it is dark in the centre or if I can see Coco through it. The whole bag is covered in mycelium and this little guy is poking up. These are the 3 best pics I can get, not easy taking pics through bag

 

[Goonie Goat]

Can anyone tell me if this is mould or mycelium? It is growing off a ridge of mycelium not sure if it is dark in the centre or if I can see Coco through it. The whole bag is covered in mycelium and this little guy is poking up. These are the 3 best pics I can get, not easy taking pics through bag

Looks like it could be some trichoderma, it's hard to say but it kind of looks greyish to me but also can't be 100% sure. Hopefully it's just Mycelium

 

[Aye Shroomer]

Can anyone tell me if this is mould or mycelium? It is growing off a ridge of mycelium not sure if it is dark in the centre or if I can see Coco through it. The whole bag is covered in mycelium and this little guy is poking up. These are the 3 best pics I can get, not easy taking pics through bag

Hard to tell. Trich usually goes green blue

 

[Orb]

Looks like it could be some trichoderma, it's hard to say but it kind of looks greyish to me but also can't be 100% sure. Hopefully it's just Mycelium

just for clarity my like was hoping it was myc - I wouldn’t have a clue on what things are meant to look like!

 

[terrancefartbubbler]

It looks like it's more of a grey green I'm pretty sure it's Coco but will find out over next few days

 

[Goonie Goat]

Still can't get over this Pink Oyster mycelium, It's so strange

Here's some healthy lion's mane mycelium

Cordyceps, healthy but bad quality because camera refocuses every second


Some examples of contamination. Coincidentally all these were from the same vendor. Not saying it's their fault that there's contamination but you can never really trust that the liquid culture is 100% clean, hence why I test them on agar before considering injecting any bag with any liquid cultures. I also don't really mind though because these LC's were from eBay and very very cheap and have been sitting in the fridge for close to 2 years. Other LCs I did on agar from a different vendor are 100% clean.

Bacterial contam on Reishi agar

Bacterial (2 spots) and fungal contam on nameko agar

Lion's mane contam (something just seems completely off with it)
Turkey tail contamination


All of this agar was dyed blue but the mycelium sucked the colouring out of it, it's exactly back to the colour it started as before adding the food colouring

 

[Orb]

real interesting Goonie, mad seeing how different myc grow, blue dye was a great idea!!

 

[Goonie Goat]

real interesting Goonie, mad seeing how different myc grow, blue dye was a great idea!!

Its slow starting up but should be cooking with gas real soon

 

[Goonie Goat]

Update. I've taken some wedges of agar from different strains and have put them into bags. Unfortunately a few got contaminated since I didn't store them properly while waiting for the agar cultures to take off. Trying a few rice pouches for the cordyceps which hopefully will work out okay

 

[Orb]

mind if I ask Goonie what went wrong storing them - I though u just put the lid on the plate and left them be and they’d be good?

 

[Goonie Goat]

mind if I ask Goonie what went wrong storing them - I though u just put the lid on the plate and left them be and they’d be good?

The agar is fine it was the bags that went bad (not yours and the others by the way), they weren't sealed up and were just sitting on the shelves clamped shut with zip ties, lost about 4 bags but the rest are fine. The rest were stored inside a storage container that was sprayed down with alcohol, no contam at all. New agar turned out great too, added 1g of Vegemite instead of yeast flakes so hopefully no weird yeasty shit starts growing on them

 

[Orb]

sometimes I reread things and wonder if English is really my first language

 

[Pikey]

sometimes I reread things and wonder if English is really my first language

An labhraionn tu Gaeilge?

 

[Goonie Goat]

Oh yeah forgot to mention, the pictures of the bags I uploaded are the good ones, that's mycelium taking off , should have been clearer with my wording, sorry about that

 

[Orb]

An labhraionn tu Gaeilge?

seah ta coupla focal agam

 

[terrancefartbubbler]

Update. I've taken some wedges of agar from different strains and have put them into bags. Unfortunately a few got contaminated since I didn't store them properly while waiting for the agar cultures to take off. Trying a few rice pouches for the cordyceps which hopefully will work out okay
View attachment 45907View attachment 45908View attachment 45909

I inoculated 4 rice pouches around 2 weeks ago they all look and smell good no shit smells or mould that I can see, might do a break and shake in a few days

 

[Goonie Goat]

I inoculated 4 rice pouches around 2 weeks ago they all look and smell good no shit smells or mould that I can see, might do a break and shake in a few days

They look great! Did you do it under a still air box??