Okay well it seems like all the jars of cultures are doing great, mycelium has multiplied very fast since the day of inoculation.
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Bad pics, hard to capture mycelium but there's an obvious difference in the amounts since I first injected.
Notice others have basically the same setup with the syringe filters after doing some research.
A list of parts for anyone interested.
Find {productName} at Bunnings. Visit your local store for the widest range of products.
www.bunnings.com.au
Shop 3 Preserving Jars - 550ml at Kmart. Enjoy Australia-wide delivery or free click & collect on selected items.
www.kmart.com.au
Use to install airline on system. Drill size hole and install grommet of your size. Push airline through to create watertight seal.
happyhydroponics.com.au
::.Generation-Preserving.:: • 100% Original • High Quality • Fast Shipping 5 SYRINGE FILTER 0.22um 13mm DIAMETER PVDF MEMBRANE 5 x Syringe Filter for Liquid Culture jars and many other uses .22um filter Material PTFE so can be autocalved 13mm Diameter
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And some 3-4mm vinyl tubing, cut to about a cm to push onto the ends of the syringe filters, so they can be jammed into the lid grommet for a tight seal.
Use an 18 gauge needle for inoculating, and for collecting use a 16 to 14 gauge needle that's big enough to slurp up the mycelium.
Generic recipe for liquid culture online
- 1500 ml water
- 30ml of glucose syrup (corn syrup)
- 2.5 grams of peptone
- 3.7 grams of light malt extract
mix well on the stove, chuck it into the jars with lids tightened loosely.
Pressure cook for 15-20mins.
Tighten lids well when finished, wait to cool and shoot up