vostok
Blooming
- User ID
- 156
Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa.
Abstract
Cannabis seed germination is an important process for growers and researchers alike. Many biotechnological applications require a reliable sterile method for seed germination. This protocol outlines a seed germination procedure for Cannabis sativa using a hydrogen peroxide (H2O2) solution as liquid germination media.
In this protocol, all three steps including seed sterilization, germination, and seedlings development were carried out in an H2O2 solution of different concentrations; 1% H2O2 solution showed the fastest and the most efficient germination. This protocol also exhibited high germination efficiency for very old cannabis seeds with lower viability. Overall, this protocol demonstrates superior germination compared to water control and reduces the risk of contamination, making it suitable for tissue culture and other sensitive applications...here: https://bio-protocol.org/e3875
Back in the day of early weed growing I use to add a drop of household bleach to my germming water in the belief I was killing any mold(dampen off) spores
and got about 50/50 good germs, 20 years back I cut that out seeing the bleach would screw the ph of the germming water, and kept a close count as one does when germming expensive seeds,
and from then on I scrapped the bleach altogether and got a sudden rise of good germs, over 80% at least ...and now I get sent this report ...so whats your story?
Abstract
Cannabis seed germination is an important process for growers and researchers alike. Many biotechnological applications require a reliable sterile method for seed germination. This protocol outlines a seed germination procedure for Cannabis sativa using a hydrogen peroxide (H2O2) solution as liquid germination media.
In this protocol, all three steps including seed sterilization, germination, and seedlings development were carried out in an H2O2 solution of different concentrations; 1% H2O2 solution showed the fastest and the most efficient germination. This protocol also exhibited high germination efficiency for very old cannabis seeds with lower viability. Overall, this protocol demonstrates superior germination compared to water control and reduces the risk of contamination, making it suitable for tissue culture and other sensitive applications...here: https://bio-protocol.org/e3875
Back in the day of early weed growing I use to add a drop of household bleach to my germming water in the belief I was killing any mold(dampen off) spores
and got about 50/50 good germs, 20 years back I cut that out seeing the bleach would screw the ph of the germming water, and kept a close count as one does when germming expensive seeds,
and from then on I scrapped the bleach altogether and got a sudden rise of good germs, over 80% at least ...and now I get sent this report ...so whats your story?